Papers

Research funded by BTRC has produced numerous publications and presentations: here is a list of the published papers that our grants have facilitated

2016 Tumor Metabolism, the Ketogenic Diet and b-Hydroxybutyrate: Novel Approaches to Adjuvant Brain Tumor Therapy

Published in Frontiers in Molecular Neuroscience 16 November 2016

Authors: Eric C. Woolf 1,2, Nelofer Syed 3 and Adrienne C. Scheck 1,2*

1 Neuro-Oncology Research, Barrow Brain Tumor Research Center, Barrow Neurological Institute, St. Joseph’s Hospital and  Medical Center, Phoenix, AZ, USA, 2 School of Life Sciences, Arizona State University, Tempe, AZ, USA, 3 The John Fulcher Molecular Neuro-Oncology Laboratory, Division of Brain Sciences, Imperial College London, London, UK

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2018 Efficacy of arginine depletion by ADI-PEG20 in an intracranial model of GBM

Published in the Official journal of the Cell Death Differentiation Association by Springer Nature CDD Press.

Authors Justyna Magdalena Przystal1,2, Nabil Hajji2, Combiz Khozoie2, Alexander Renziehausen2, Qingyu Zeng2,  Fernando Abaitua2, Amin Hajitou1, Keittisak Suwan1, ElizabethWant3, John Bomalaski4, Peter Szlosarek5, Kevin O’Neill2,  Tim Crook6 and Nelofer Syed2

Correspondence: Nelofer Syed (n.syed@imperial.ac.uk)  1Phage Therapy Group, Division of Brain Sciences, Imperial College London,  London, UK  2John Fulcher Neuro-Oncology Laboratory, Division of Brain Sciences, Imperial  College London, London, UK.  Full list of author information is available at the end of the article.  These authors contributed equally: Justyna Magdalena Przystal, Nabil Hajji  Edited by A. Stephanou

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2018 The renin angiotensis system_oncogene

“The renin angiotensin system (RAS) mediates bifunctional growth regulation in melanoma and is a novel target for therapeuticintervention” – Published online 26 November 2018 in nature.com

The work was supported by The Chief Scientific Officer of Scotland, The Anonymous Trust, Tenovus Scotland (to Dr. T.C.), Melanoma Focus (to C.P.), The Leng Foundation (to C.P.) and The Brain Tumour Research Campaign (to N.S.). Antonio Vega-Rioja is under contract Proyectos I + D + I para jovenes investigadores from de Economica y Competitividad (SAF2014–60649-JN) and co-funded by Fondo Europeo de Regional-FEDER. Tim Crook is a Scottish Senior Clinical Fellow in Medical Oncology.

These authors contributed equally: Alexander Renziehausen, Andreas G. Tzakos, Nelofer Syed, Tim Crook.

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2018 Development of a validated LC-MS-MS method for the in vitro and in vivo quantitation of sunitinib

“Development of a validated LC-MS/MS method for the in vitro and in vivo quantitation of sunitinib in glioblastoma cells and cancerpatients” – Published in the Journal of Pharmaceutical and Biomedical Analysis 12 November 2018

Authors: a:Maria V. Chatziathanasiadoua,1, Evgenios K. Stylos a,b,1, Efstathia Giannopoulouc,  Maria-Helen Spyridaki d, Evangelos Briasoulis e, Haralabos P. Kalofonos c, Tim Crook f
, Nelofer Syed g, Gregory B. Sivolapenko h, Andreas G. Tzakos a,e,∗

a Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45110, Ioannina, Greece b Biotechnology Laboratory, Department of Biological Applications and Technology, University of Ioannina, Ioannina, 45110, Greece c Clinical Oncology Laboratory, Division of Oncology, Department of Medicine, University of Patras, 26504, Rio, Greece d General Chemical State Laboratory, Chemical Service of Peloponnese, Western Hellas and Ionian Islands, Department of Chemical Services of Corfu, Greece e Cancer Biobank Center and Department of Hematology, School of Medicine, University of Ioannina, 45110, Ioannina, Greece f St Luke’s Cancer Centre, Royal Surrey County Hospital, Guildford, Surrey, UK g John Fulcher Neuro-Oncology Laboratory, Imperial College London, Hammersmith Hospital, London, UK h Department of Pharmacy, Laboratory of Pharmacokinetics, University of Patras, Patras, Greece

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2017 Global Proteome and Phospho-proteome Analysis of Merlin-deficient Meningioma and Schwannoma

“Global Proteome and Phospho-proteome Analysis of Merlin-deficient Meningioma and Schwannoma Identifies PDLIM2 as a NovelTherapeutic Target” – Published in EBioMedicine 13 January 2017

Authors: Kayleigh Bassiri a,1, Sara Ferluga a,1, Vikram Sharma b, Nelofer Syed c, Claire L. Adams a,Edwin Lasonder b, C Oliver Hanemann a,

⁎a Institute of Translational and Stratified Medicine, Plymouth University Peninsula Schools of Medicine and Dentistry, John Bull Building, Plymouth Science Park, Research Way, Derriford, Plymouth PL6 8BU, UK
b School of Biomedical and Healthcare Sciences, Plymouth University, Drakes Circus, Plymouth PL4 8AA, UK
c John Fulcher Neuro-oncology Laboratory, Division of Brain Sciences, Faculty of Medicine, Imperial College London, London W6 8RP, UK

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2018 Development and validation of simple step protein precipitation

“Development and validation of simple step protein precipitation UHPLC-MS/MS methods for quantitation of temozolomide in cancer patient plasma samples”  Published in Journal of Pharmaceutical and Biomedical Analysis 7th September 2018

Authors: Mohamed A. El Mubaraka,1, Evgenios K. Stylos b,c,1, Maria V. Chatziathanasiadoub, Charikleia Danikaa, George A. Alexioud, Periklis Tsekeris e, Alexander Renziehausenf , Tim Crook g, Nelofer Syed f , Gregory B. Sivolapenko a,∗, Andreas G. Tzakos b,∗

*a Department of Pharmacy, Laboratory of Pharmacokinetics, University of Patras, Patras, Greece  b Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, GR-45110, Ioannina, Greece  c Biotechnology Laboratory, Department of Biological Applications and Technology, University of Ioannina, Ioannina, 45110, Greece d Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece  e Department of Radiation Oncology, University Hospital of Ioannina, Ioannina, Greece  f John Fulcher Neuro-Oncology Laboratory, Imperial College London, Hammersmith Hospital, London, UK  g St Luke’s Cancer Centre, Royal Surrey County Hospital, Guildford, Surrey, UK

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2018 Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM

” Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM” Published online in BMC Cancer 7th February 2018

Authors: Lina Mörén 1, Richard Perryman 2 , Tim Crook 3, Julia K. Langer 2, Kevin O’Neil 2, Nelofer Syed 2* and Henrik Antti 1*

1 Department of Chemistry, Umeå University, SE 901 87 Umeå, Sweden.2 John Fulcher Neuro-Oncology Laboratory, Imperial College London, London, UK. 3 St Luke’s Cancer Centre, Royal Surrey County Hospital, Guildford, Surrey, UK.

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2018 The S100A4 Protein Signals through the ErbB4 Receptor to Promote Neuronal Survival

“The S100A4 Protein Signals through the ErbB4 Receptor to Promote Neuronal Survival”  Published in Theranostics 2018, Vol. 8, Issue 14 1 July 2018

Authors: Stanislava Pankratova 1,2, Jorg Klingelhofer 1, Oksana Dmytriyeva 1,3, Sylwia Owczarek 1, Alexander Renziehausen 4, Nelofer Syed 4, Alexandra E. Porter 5, David T. Dexter 6, and Darya Kiryushko 5,6  Corresponding author: d.kiryushko@imperial.ac.uk
1. Laboratory of Neural Plasticity, Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen, Denmark
2. Department of Pediatrics and Adolescent Medicine, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark
3. Research Laboratory for Stereology and Neuroscience, Bispebjerg Hospital, Bispebjerg Bakke 23, 2400 Copenhagen, Denmark
4. John Fulcher Neuro-Oncology Laboratory, Imperial College London, Hammersmith Hospital Campus, Burlington Danes Building, 160 Du Cane Road, W12 0NN London, UK
5. Department of Materials and London Center for Nanotechnology, Imperial College, Exhibition Road, SW72AZ London, UK
6. Center for Neuroinflammation and Neurodegeneration, Imperial College London, Hammersmith Hospital Campus, Burlington Danes Building, 160 Du Cane Road, W12 0NN London, UK

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Visualising the Vasculature of a Human Glioma in Mouse Brain using CLARITY

Citation: Liu AKL, Przystal JM, Lai HM, et al. Visualising the Vasculature of a Human Glioma in Mouse Brain using CLARITY. Arch Cancer Res. 2016, 4:1.

Alan King Lun Liu 1, Jusytna M Przystal 1, Hei Ming Lai 2, Kevin O’Neill 1 and Nelofer Syed 1

1: Division of Brain Sciences, Department of Medicine, Imperial College London, UK

2: State Key Laboratory of Brain and Cognitive Sciences, School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong

Bacteriophage-Derived Vectors for Targeted Cancer Gene Therapy

Viruses 2015, 7, 268-284; doi:10.3390/v7010268

Md Zahidul Islam Pranjol 1 and Amin Hajitou 2,*

1: Institute of Clinical and Biomedical Science, University of Exeter Medical School, Exeter, Devon EX1 2LU, UK; E-Mail: z.pranjol@exeter.ac.uk

2: Phage Therapy Group, Department of Medicine, Burlington Danes Building, Imperial College London, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK

Bacteriophage Mediates Efficient Gene Transfer in Combination with Conventional Transfection Reagents

Viruses Received: 19 August 2015; Accepted: 1 December 2015; Published: 8 December 2015

Academic Editor: Abram Aertsen

Amanda Donnelly 1,†, Teerapong Yata 1,2,†, Kaoutar Bentayebi 3, Keittisak Suwan 1 and Amin Hajitou 1, *

1: Phage Therapy Group, Division of Brain Sciences, Department of Medicine, Imperial College London, Hammersmith Hospital Campus, London W12 0NN, UK; donnela1@tcd.ie (A.D.); teerapong@nanotec.or.th (T.Y.); keittisak.suwan@imperial.ac.uk (K.S.)

2: National Nanotechnology Center, National Science and Technology Development Agency, 111 Thailand Science Park, Pathum Thani 12120, Thailand

3: Laboratory of Genetics, University of Balearic islands, Valldemossa Road Km. 7,5, 07122 Palma, Spain; kaoutar.bentayebi@gmail.com

Modulation of extracellular matrix in cancer is associated with enhanced tumor cell targeting by bacteriophage vectors

Published in Molecular Cancer June 2015

Citation © 2015 Yata et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

Teerapong Yata 1,2†, Eugene L. Q. Lee 1†, Keittisak Suwan 1†, Nelofer Syed 3, Paladd Asavarut 1 and Amin Hajitou 1,4

Hybrid Nanomaterial Complexes for Advanced Phage-guided Gene Delivery

Citation: Molecular Therapy—Nucleic Acids (2014) 3, e185; doi:10.1038/mtna.2014.37

© 2014 The American Society of Gene & Cell Therapy

Teerapong Yata1, Koon-Yang Lee 2, Tararaj Dharakul 3, Sirirurg Songsivilai 3, Alexander Bismarck 2, Paul J Mintz 4 and Amin Hajitou 1

Epigenetic status of argininosuccinate synthetase and argininosuccinate lyase modulates autophagy and cell death in glioblastoma

Citation: Cell Death and Disease (2013) 4, e458; doi:10.1038/cddis.2012.197 & 2013 Macmillan Publishers Limited All rights reserved 2041-4889/13

N Syed *,1, J Langer 1, K Janczar 1, P Singh 1, C Lo Nigro 2, L Lattanzio 2, HM Coley 3, E Hatzimichael 4, J Bomalaski 5, P Szlosarek 6, M Awad 7, K O’Neill 7, F Roncaroli 1 and T Crook 8

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Dual systemic tumor targeting with ligand-directed phage and Grp78 promoter induces tumor regression

Published in final edited form as Mol Cancer Ther. 2012 December ; 11(12): 2566–2577. doi:10.1158/1535-7163.MCT-12-0587.

Azadeh Kia 1, Justyna M. Przystal 1, Nastasia Nianiaris 1, Nicholas D. Mazarakis 1, Paul J. Mintz 2, and Amin Hajitou 1

1: Gene Therapy, Division of Brain Sciences, Department of Medicine, Imperial College London, Hammersmith Hospital Campus, London W12 0NN, UK

2: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London W12 0NN, UK

Proteasome inhibition in cancer is associated with enhanced tumor targeting by the adeno-associated virus/phage

Molecular Oncology XXX (2012) 1-12 August 01, 2012  

Justyna M. Przystal a,1, Eloho Umukoro a,1, Charlotte A. Stoneham a, Teerapong Yata aKevin O’Neill b, Nelofer Syed b, Amin Hajitou a

a: Division of Brain Sciences, Hammersmith Hospital Campus, Department of Medicine, Imperial College London, Burlington Danes Building, 160 Du Cane Road, London W12 0NN, United Kingdom

b: Division of Brain Sciences, Charing Cross Campus, Department of Medicine, Imperial College London, London, United Kingdom

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Site-specific CpG methylation in the CCAAT/enhancer binding protein delta (CEBPδ) CpGisland in breast cancer is associated with metastatic relapse

Published in British Journal of Cancer. 2012 Jul 10

Palmieri C, Monteverde M, Lattanzio L, Gojis O, Rudraraju B, Fortunato M, Syed N, Thompson A, Garrone O, Merlano M, Lo Nigro C, CrookT.

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NT5E CpG island methylation is a favourable breast cancer biomarker

Br J Cancer. 2012 Jun 26; 107(1): 75–83. Published online 2012 May 31. doi:  10.1038/bjc.2012.212

PMCID: PMC3389412

C Lo Nigro,1,10 M Monteverde,1,10 S Lee,2,10 L Lattanzio,1 D Vivenza,1 A Comino,3 N Syed,4 A McHugh,5 H Wang,5 C Proby,5 O Garrone,6 M Merlano,6 E Hatzimichael,7 E Briasoulis,7 O Gojis,8 C Palmieri,8 L Jordan,9 P Quinlan,9 A Thompson,9 and T Crook9

Methylation of the calcium channel regulatory subunit α2δ-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas

Br J Cancer. 2012 Jul 10; 107(2): 375–381.

Published online 2012 May 29. doi:  10.1038/bjc.2012.231

PMCID: PMC3394973

C Palmieri,1,* B Rudraraju,1 M Monteverde,2 L Lattanzio,2 O Gojis,1,3,4 R Brizio,5 O Garrone,6 M Merlano,6 N Syed,7 C Lo Nigro,2,9 and T Crook8,9

NT5E (CD73) is epigenetically regulated in malignant melanoma and associated with metastatic site specificity

Br J Cancer. 2012 Apr 10; 106(8): 1446–1452. Published online 2012 Mar 27. doi:  10.1038/bjc.2012.95 PMCID: PMC3326678

H Wang,1 S Lee,2 C Lo Nigro,3 L Lattanzio,3 M Merlano,3 M Monteverde,3 R Matin,4 K Purdie,4 N Mladkova,4 D Bergamaschi,4 C Harwood,4 N Syed,5 P Szlosarek,6 E Briasoulis,7 A McHugh,1 A Thompson,8 A Evans,1 I Leigh,1 C Fleming,1 G J Inman,8 E Hatzimichael,7 C Proby,1 and T Crook8,*

High frequency of complex TP53 mutations in CNS metastases from breast cancer

Br J Cancer. 2012 Jan 17; 106(2): 397–404.

Published online 2011 Dec 20. doi:  10.1038/bjc.2011.464

PMCID: PMC3261685

C Lo Nigro,1,8 D Vivenza,1,8 M Monteverde,1 L Lattanzio,1 O Gojis,2,7 O Garrone,3 A Comino,4 M Merlano,3 P R Quinlan,5 N Syed,6 C A Purdie,5 A Thompson,5 C Palmieri,2 and T Crook5,*

Intra-operative, real-time, three-dimensional ultrasound assisted positioning of catheters in the microdialysis of glial tumours

Journal of Clinical Neuroscience 2010, doi:10.1016/j.jocn.2009.06.022.  Bob Homapour a, Jonathan E. Bowen b, Elizabeth J. Want b, Kevin O’Neill a, Vasileios Apostolopoulos a, Dipankar Nandi a, James R. Van Dellen a, Federico Roncaroli a,*

a: Department of Clinical Neuroscience, Division of Neuroscience and Mental Health, Imperial College, Imperial Healthcare NHS Trust and BTRC Research Group, Charing Cross Campus, St Dunstans Road, London W6 8RP, UK

b: Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Imperial College, London, UK

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Launching a Novel Preclinical Infrastructure: Comparative Oncology Trials Consortium Directed Therapeutic Targeting of TNFa to Cancer Vasculature

Citation: Paoloni MC, Tandle A, Mazcko C, Hanna E, Kachala S, et al. (2009) Launching a Novel Preclinical Infrastructure: Comparative Oncology Trials Consortium

Directed Therapeutic Targeting of TNFa to Cancer Vasculature. PLoS ONE 4(3): e4972. doi:10.1371/journal.pone.0004972

Melissa C. Paoloni 1, Anita Tandle 2, Christina Mazcko 1, Engy Hanna 2, Stefan Kachala 2, Amy LeBlanc 3, Shelley Newman 3, David Vail 4, Carolyn Henry 5, Douglas Thamm 6, Karin Sorenmo 7, Amin Hajitou 8, Renata Pasqualini 9, Wadih Arap 9, Chand Khanna 1.*, Steven K. Libutti 2

Intraosseous Angiolipoma of the Cranium: Case Report

Published in Neurosurgery Volume 64 Number 1 January 2009  Kenny Yu, M.D., James Van Dellen, M.D., Philip Idaewor, M.D., Federico Roncaroli, M.D.

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Galectin-3 Expression is Ubiquitous in Tumors of the Sellar Region, Nervous System, and Mimics

An Immunohistochemical and RT-PCR Study

Fausto J. Rodriguez, MD,* Bernd W. Scheithauer, MD,* Federico Roncaroli, MD, Ana I. Silva, MD, Kalman Kovacs, MD, PhD,y Daniel J. Brat, MD, PhD,J and Long Jin, MD*

Published in American Journal of Surgery and Pathology, Volume 00, Number 00, 2008

The lack of expression of the peripheral benzodiazepine receptor characterises microglial response in anaplastic astrocytomas

Published in Journal of Neurooncology  DOI 10.1007/s11060-007-9396-1

Received: 26 January 2007 / Accepted: 23 April 2007  Springer Science+Business Media B.V. 2007

Shigetoshi Takaya, Kazuo Hashikawa, Federico E. Turkheimer, Nicholas Mottram,Manuel Deprez, Koichi Ishizu, Hidekazu Kawashima, Haruhiko Akiyama, Hidenao Fukuyama, Richard B. Banati, Federico Roncaroli

Papillary Tumor of the Pineal Region and Spindle Cell Oncocytoma of the Pituitary: New Tumor Entities in the 2007 WHO Classification

Published in Brain Pathology; issue 17:314–318, 2007  Federico Roncaroli, University Department of Neuropathology, Imperial College, London, UK. Bernd W. Scheithauer, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.

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Microglia in Gemistocytic Astrocytomas

Published in Neurosurgery Volume 60 Number 1 January 2007F. Geranmayeh, B.Sc., M.B.B.S., B.W. Scheithauer, M.D., C. Spitzer, M.D., F.B. Meyer, M.D., A.C. Svensson-Engwall, Ph.D., Manuel B. Graeber, M.D., Ph.D.

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Chromosomal patterns of gene expression from microarray data: methodology, validation and clinical relevance in gliomas

Federico E Turkheimer1, Federico Roncaroli2, Benoit Hennuy3, Christian Herens3, Minh Nguyen4, Didier Martin4, Annick Evrard3, Vincent Bours3, Jacques Boniver5 and Manuel Deprez5

Published in BMC Bioinformatics20067:526 DOI: 10.1186/1471-2105-7-526 ©  Turkheimer et al; licensee BioMed Central Ltd. 2006

Published: 01 December 2006

A Hybrid Vector for Ligand-Directed Tumor Targeting and Molecular Imaging

Published in Cell DOI 10.1016/j.cell.2006.02.042

Amin Hajitou,1 Martin Trepel,5,6 Caroline E. Lilley,7 Suren Soghomonyan,2 Mian M. Alauddin,2 Frank C. Marini, III,3 Bradley H. Restel,1 Michael G. Ozawa,1 Catherine A. Moya,1 Roberto Rangel,1 Yan Sun,1 Karim Zaoui,5,6 Manfred Schmidt,5,6 Christof von Kalle,5,6 Matthew D. Weitzman,7 Juri G. Gelovani,2 Renata Pasqualini,1,4,*and Wadih Arap1,4